Acoustophoretic cell processing in medical applications
Time: 2013.07.01 9:30am
Address: Rm. 103, Micro/nanoelectronics building, Peking University
Speaker: Thomas Laurell
Dept. Measurement Sci. Ind. Electronics
Microchip acoustophoresis have in recent years become a viable strategy for advanced cell manipulation in life science applications. Convincing evidence now also exists, demonstrating that cells can undergo microchip acoustophoresis without any signs of altered viability. This now opens the route for a wide range of bioanalytical and medical applications.
Utilising the fact that different cell types display individual acoustophoretic mobility enables the use of Free Flow Acoustophoresis, FFA, to separate different cell types in a continuous flow. A recently developed FFA-platform to address the clinical needs for label free extraction tumor cells will be presented. FFA has also been investigated as a means to improve peripheral blood stem cell (PBSC) extraction in transfusion medicine where platelet rich apheresis products pose a severe problem in the subsequent immunomagnetic extraction of stem cells. We demonstrate that acoustophoresis can offer efficient platelet reduction without compromising the PBSC collection.
In line with these developments acoustic trapping has more recently emerged as an important modality, which allows non-contact retention of cells and particles in continuous flows systems. This enables simple means of chip integrated washing of beads or cells and on-line incubation and buffer switching. Acoustic trapping also offers enrichment of particles/cells in dilute suspensions. The primary acoustic radiation force scales with the volume of the particle and hence as we move towards smaller biological particles a typical limit of operation is in the range of 2 um. Our recent developments in acoustic trapping have however found ways to circumvent this, which has opened the route to enrichment of bacteria and submicron particles.
Thomas Laurell is Professor in Medical and Chemical Microsensors at Lund University. Laurell has a background in engineering with a focus on biomedical technology, with a PhD from the technical faculty at Lund University. Laurell has > 20 years experience in the development of lab-on-a-chip based bioanalytical and medical diagnostic technology. He was appointed distinguished professor at dept. Biomedical Engineering at Dongguk University, Seoul, Korea in 2009.
Laurell is a member of Royal Swedish Academy of Sciences, of Royal Swedish Academy of Engineering Sciences and Royal Physiographic Society. He has received several prestigious awards such as the The AkzoNobel Science Award in 2009 for his ground breaking interdisciplinary research, Wallmark Award by The Royal Swedish Academy of Sciences for his microsensorsystem innovations in 2006, SKAPA Award in memory of Alfred Nobel ? for the best innovation of the year in Sweden 1996 and 2003, and Erna Ebelings Price for outstanding research in microfluidics and biomedical engineering in 2006. He is founder of four start-up companies. Laurell is also the President of the Chemical and Biological Microsystems Society (www.cbmsociety.org).